Figure 4From: Vaccinia virus A12L protein and its AG/A proteolysis play an important role in viral morphogenic transitionMorphology defects by abrogated AG/A cleavage of A12L. In order to examine VV morphology by rescuing the absence of A12L, we transfected plasmid born A12L under the control of an early/late synthetic promoter (pA12L) and AG/A mutant plasmid of A12L (AG/A), and infected with vvtetO A12L in the absence of Tet. The transient expression of A12L induced regular IV and IMV particles (panel a) while the AG/A mutation into ID/I displayed defective phenotypes (panel b through d). Arrows in panel a indicate center-concaved inner layer of the core. Panel b and c show IV particles with little or almost empty viral materials while panel d demonstrates the aberrant layers of the cores.Back to article page