Figure 1

Tet-dependent replication of vvtetO A12L and one-step growth curve. a. Tet-dependent replication of vvtetO A12L. T-REx 293 cells were infected with vvtetO A12L at an MOI of 1 PFU/cell in the presence of tetracycline (Tet) at various concentrations of 0, 10, 20, 30, and 40 μg/mL. The infected cell extracts harvested at 24 hpi were titered on BSC 40 cells to determine the virus yields. b. One-step growth curve. T-REx 293 cells were infected with vvtetO A12L in the presence and absence of Tet (30 μg/mL) and harvested at 3, 5, 8, 12, and 24 hpi. Each virus titer (PFU/ml) was scaled in log phase.