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Figure 4 | Virology Journal

Figure 4

From: Repressor of temperate mycobacteriophage L1 harbors a stable C-terminal domain and binds to different asymmetric operator DNAs with variable affinity

Figure 4

Equilibrium binding and kinetic study. Equilibrium binding of CI to O64 (A) and OL (B) operators was studied according to standard method as described in Materials and methods. Plots of % operator bound (estimated from inset gel shift assay pictures) versus CI concentration (0.05 – 0.45 μM and 0.1 – 0.8 μM CI with O64 and OL, respectively) are shown. Nearly 0.1 nM labeled operator was used in each reaction. (C) Plot of %operator bound versus time shows the kinetics of CI dissociation from O64 and OL operators in presences of excess cold operator. The amount of operator bound in the shifted complex of the zero time aliquot was considered as 100%. (D) Plot of log Keq versus 1/T shows equilibrium binding of CI to O64 operator at temperatures ranging from 25° – 42°C. All curves/lines are best-fit curves/lines.

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