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Table 1 Checkerboard titration assay for measuring the blocking activity of anti-CD151 Ab

From: Role of CD151, A tetraspanin, in porcine reproductive and respiratory syndrome virus infection

Virus dilution (1:9-diluted) ↓ 10-1 C C C 0 0 1 2.5 3 3 3 3 3
  10-2 C c c 0 0 1 2.5 3 3 3 3 3
  10-3 C c c 0 0 0.5 1 2 2 2 2 2
  10-4 c c c 0 0 0 0 0.5 1 1 1 1
  10-5 c c c 0 0 0 0 0 0.5 0.5 0.5 0.5
  10-6 c c c 0 0 0 0 0 0 0 0 0
  10-7 c c c 0 0 0 0 0 0 0 0 0
  No virus C c c 0 0 0 0 0 0 0 0 0
   Ab dilution(1:1-diluted) → No Ab
   20-1 40-1 80-1 160-1 320-1 640-1 1280-1 2560-1 5120-1    
  1. MARC-145 cells were cultured with polyclonal anti-CD151 Ab and/or PRRSV in a 96-well tissue culture plate. Polyclonal anti-CD151 Ab was 1:1-serially diluted from 20-1-dilution, and the PRRSV preparation was 1:9-serially diluted from 10-1-dilution. At 2 days post infection, immunofluorescence microscopy analysis was performed. The cells were stained with FITC-conjugated SDOW-17, a MAb against PRRSV nucleocapsid protein. The cells were examined by fluorescent microscopy. C means the cytopathic effect of Ab, and the numbers mean the intensity of fluorescence (0 means no fluorescence detected, and 3 means the highest intensity of fluorescence)