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Table 1 Checkerboard titration assay for measuring the blocking activity of anti-CD151 Ab

From: Role of CD151, A tetraspanin, in porcine reproductive and respiratory syndrome virus infection

Virus dilution (1:9-diluted) ↓

10-1

C

C

C

0

0

1

2.5

3

3

3

3

3

 

10-2

C

c

c

0

0

1

2.5

3

3

3

3

3

 

10-3

C

c

c

0

0

0.5

1

2

2

2

2

2

 

10-4

c

c

c

0

0

0

0

0.5

1

1

1

1

 

10-5

c

c

c

0

0

0

0

0

0.5

0.5

0.5

0.5

 

10-6

c

c

c

0

0

0

0

0

0

0

0

0

 

10-7

c

c

c

0

0

0

0

0

0

0

0

0

 

No virus

C

c

c

0

0

0

0

0

0

0

0

0

  

Ab dilution(1:1-diluted) →

No Ab

  

20-1

40-1

80-1

160-1

320-1

640-1

1280-1

2560-1

5120-1

   
  1. MARC-145 cells were cultured with polyclonal anti-CD151 Ab and/or PRRSV in a 96-well tissue culture plate. Polyclonal anti-CD151 Ab was 1:1-serially diluted from 20-1-dilution, and the PRRSV preparation was 1:9-serially diluted from 10-1-dilution. At 2 days post infection, immunofluorescence microscopy analysis was performed. The cells were stained with FITC-conjugated SDOW-17, a MAb against PRRSV nucleocapsid protein. The cells were examined by fluorescent microscopy. C means the cytopathic effect of Ab, and the numbers mean the intensity of fluorescence (0 means no fluorescence detected, and 3 means the highest intensity of fluorescence)