Figure 3
From: Role of CD151, A tetraspanin, in porcine reproductive and respiratory syndrome virus infection
Detection of the presence of CD151 by RT-PCR and Western blot. Correlation between CD151 expression and susceptibility to PRRSV infection was demonstrated by RT-PCR and Western blot analysis. (A) RT-PCR showing the amplification of 105 bp amplicon with CD151-specific primers was performed for RNAs isolated from PRRSV-susceptible and -non-susceptible cell lines. M, 123 bp ladder; lane 1, negative RT control; lane 2, negative PCR control; lane 3, HRT; lane 4, MARC-145; lane 5, MDBK; lane 6, BHK-21; lane 7, ST; lane 8, MA-104; lane 9, ST-K; lane 10, Vero; lane 11, CL-2621; lane 12, COS; lane 13, CD151-transfected BHK-21. (B) Western blot analysis using anti-CD151 MAb was performed for cell lysates from PRRSV-susceptible and -non susceptible cell lines. Lane 1, MARC-145; lane 2, BHK-21; lane 3, Vero. (C) Flow cytometric analysis using polyclonal anti-CD151 Ab was performed for MARC-145 (C (1)) and BHK-21 (C (2)) cell lines. An isotype-matched control is represented by the dotted lines.