Figure 3
From: Porcine adenovirus type 3 E1Blarge protein downregulates the induction of IL-8
EMSA of nuclear extracts. (A). Nuclear extracts from the plasmid transfected cells (I, II, III) incubated with radiolabeled oligonucleotide probe(s) containing wild-type (I, II) or mutant (III) NF-κB motif from human IL-8 promoter [30] with (II) or without (I) immunoprecipitation with anti- NF-κB p65 serum. (B) Nuclear extracts from mock infected or virus infected cells containing wild-type (I) or mutant (II) NF-κB motif from human IL-8 promoter. (C) Schematic diagram showing deletion of the regions in PAV211, PAV212 and PAV227 [19,20].