Figure 4From: HIV-1 Vpr activates the G2 checkpoint through manipulation of the ubiquitin proteasome systemVpr(R80A) functions as a dominant-negative molecule. HeLa cells were infected with a constant MOI (MOI = 1) of pHR-VPR vector, and a variable MOI (1, 0.5, 0.25) of pHR-GFP, pHR- Vpr(R80A) or pHR-VPR(Q65R, R80A) as indicated. Cell cycle profiles were evaluated at 48 hours post transduction.Back to article page