Distribution of LCMV in the brain and spleen following an intravenous clone 13 infection. Representative sagittal brain and spleen reconstructions (n = 3 mice per group) were assembled at the denoted time points post-infection to reveal the distribution of LCMV (green) following an intravenous infection with 2 × 106 PFU of clone 13. Note the minimal amount of virus in the brain at day 10 and the complete inundation of the brain parenchyma by day 30. During the late phase of persistence (day 150), clone 13 localizes primarily to the olfactory bulb (white arrow) and also maintains a presence in the meninges, choroid plexus, ependyma, and subventricular zone. Note that the spleen shows the highest viral antigen load at day 10 and is progressively purged of virus over time. Cell nuclei are shown in blue.