Detection of virus mRNA within infected host cells using an isothermal nucleic acid amplification assay: marine cyanophage gene expression within Synechococcus sp

Table 1 Oligonucleotide probe sequences used in this study.

	Cyanophage target
	S-PM2 g20 coding strand	S-PM2 g20 non-coding strand
Extension probe	TGACCATCGTAAACAAGCTT GTTTCTGTATTCGAAAT	AACAATACTTGCGTGATGTAAT GTCACGTTTTCGAAAT
Template probe	TCGTCTTCCGGTCTCTCCTCT CAAGCCTCAGCGCTCTCTCTC CCTATAGTGAGTCGTATTAATT TCGAAhACGTGACATTACATCA CGCAAGTATTGTTx	TCGTCTTCCGGTCTCTCCTCTCA AGCCTCAGCGCTCTCTCTCCCT ATAGTGAGTCGTATTAATTTCGA AhACAGAAACAAGCTTGTTTACG ATGGTCAAx
Facilitator 1	TGCTTTTTATCATCACGAATC TCTCCTGTTx	ATGTTGGTAATCTACCAAAGGTA AAGGCAGx
Facilitator 2	CTGCCTTTACCTTTGGTAGA TTACCAACAx	ACAGGAGAGATTCGTGATGATAA AAAGCATx

All sequences are written (5' → 3').
S-PM2 GenBank accession number AF016384.
h Indicates position of hexaethylene glycol linker molecule.
x Indicates position of phosphorylation to prevent extension.
Oligonucleotides used for further amplification and detection of the RNA signal are described in Hall et al. [4].

ISSN: 1743-422X