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Figure 2 | Virology Journal

Figure 2

From: Detection of virus mRNA within infected host cells using an isothermal nucleic acid amplification assay: marine cyanophage gene expression within Synechococcus sp

Figure 2

Specific detection of cyanophage S-PM2 g20 target RNA or DNA extracted from infected host Synechococcus sp. WH7803. Graphs show signals generated from probes targeting either the coding strand (a) (to detect DNA + RNA) or non-coding strand (b) (to detect DNA but not RNA). RNA and DNA was extracted from infected cultures grown in flask 2 (24 hours prior to culture lysis). Results are compared to signals generated by both sets of probes using nucleic acid extracted from the uninfected control culture (flask 3). Graphs show the amount of RNA signal (fmol) generated from each target as determined by ELOSA.

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Virology Journal

ISSN: 1743-422X