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Figure 2 | Virology Journal

Figure 2

From: Nuclear import of influenza A viral ribonucleoprotein complexes is mediated by two nuclear localization sequences on viral nucleoprotein

Figure 2

Biotinylation of influenza vRNPs. (A) Northern blot of biotinylated influenza vRNA in the vRNP complexes. The biotinylated vRNPs were subjected through urea gel electrophoresis, transferred onto nitrocellulose, and detected by blotting with streptavidin alkaline phosphatase. Shown are the results of the same blot exposed at two different times. As a control, biotin maleimide only was run on the gel. Urea gel electrophoresis of the same biotinylated influenza vRNA shown in A. (B) The vRNA within the vRNP complex was visualized directly by staining the gels with SYBR Safe. The arrows denote the positions of the influenza vRNA. Two different concentrations of the vRNA are shown. The positions of various sizes of RNA molecular standards (in bp) are shown on the left. (C) Electron microscopy visualization of specific binding of streptavidin-gold (10-nm diameter) to biotinylated influenza vRNPs. Gold particles exclusively associated with one end of the vRNPs.

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