Figure 1
From: Efficient replication of pneumonia virus of mice (PVM) in a mouse macrophage cell line
Infection of the mouse macrophage RAW 264.7 cell line with PVM. (A) A plaque assay targeting the standard BS-C-1 primate epithelial cell line (lower panel) compared to an uninfected control cell monolayer (upper panel). (B) Western blot of infected (+pvm) and control (-pvm) RAW 264.7 cell extracts (2 × 106 cell equivalents/lane) probed with rabbit polyclonal antisera directed against a specific 15-mer of the PVM N peptide. (C) Q-RT-PCR detecting PVM SH gene per microgram RNA [13] from triplicate cultures of infected (+pvm) and uninfected (-pvm) RAW 264.7 cells demonstrating ongoing PVM replication in infected cells; *p < 0.05 as indicated. (D) Q-RT-PCR detection of interferon-β in RNA from triplicate cultures of infected (+pvm, grey bars) and uninfected control (-pvm, white bars) cells, day 3 no infection normalized to 1.0 [13]; *p < 0.02 as indicated. (E) Detection of proinflammatory cytokines (MIP-1α, MIP-1β, MIP-2 and TNF-α) in culture supernatants in response to infection, data shown with background levels subtracted; *p < 0.01 as indicated.