Figure 4

Real-time RT-PCR analysis of HPV-16 E7 mRNA. CaSki cells were infected with R3616, R3617, R5225, and R5226 at an m.o.i. of 0.1, 1.0, and 3.2, and harvested at 16 h.p.i. Total RNA was extracted and reverse transcription performed. Real-time PCR using the cDNA samples was done as described in Materials and Methods. The amount of E7 mRNA in HSV infected CaSki cells is given in copies of E7 mRNA molecules, corrected against rRNA levels in the same samples. The statistical significances of the differences in E7 copy numbers in comparison with uninfected CaSki cells are indicated by asterisks above the columns (*:p < 0.05; ***: p ≤ 0.001). The significances are shown for the cases with reduced E7 mRNA levels. The E7 mRNA values of the tk+ control R3616 were also compared with those of the tk+ antisense recombinant R5226 at the same m.o.i., and the tk- control R3617 values were compared with the tk- recombinant R5225 values at the same m.o.i.. The statistical significances in the pairwise comparisons for the reduced E7 mRNA levels are indicated by (#) above the respective columns (#: p < 0.05; ###: p ≤ 0.001).