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Figure 4 | Virology Journal

Figure 4

From: Posttranslational N-glycosylation of the hepatitis B virus large envelope protein

Figure 4

N-glycosylation within the preS domain occurs posttranslationally. The inducible EcR-LHA-293 cell line was either mock-treated (lane 1) or treated with PonA for 3 h to pulse-induce L synthesis. After PonA removal, L was chased for the indicated time periods in the presence of cycloheximide, and lysates were analyzed by SDS-PAGE and L-specific Western blotting. The nonglycosylated p39 and single-glycosylated gp42 forms as well as the newly discovered isoforms of L are indicated at the right.

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