From: Biochemical characterization of the fidelity of poliovirus RNA-dependent RNA polymerase
Sym-Sub | NTP | K d (μM) | k pol (sec-1) | k pol /K d (μM -1 sec-1) | Fidelity1 |
---|---|---|---|---|---|
U | A | 120 ± 74 | 74 ± 22 | 0.62 | - |
G2 | 1100 ± 100 | 0.004 (± 3.2) × 10-4 | 3.6 × 10-6 | 1.7 × 105 | |
U | 1.7 (± 0.53) × 105 | 0.14 ± 0.053 | 8.2 × 10-7 | 7.5 × 105 | |
C3 | 3000 | 5.4 × 10-4 | 1.8 × 10-7 | 3.4 × 106 | |
C | G | 1.6 ± 2.3 | 15 ± 9.2 | 9.4 | - |
A | 1.0 × 104 | 7.9 | 7.9 × 10-4 | 1.2 × 104 | |
U | 270 ± 120 | 0.0079 ± 0.0014 | 2.9 × 10-5 | 3.2 × 105 | |
C3 | 620 | 0.0017 | 2.7 × 10-6 | 3.4 × 106 | |
A | U | 27 ± 0.95 | 84 ± 0.48 | 3.1 | - |
C | 6.0 (± 0.70) × 105 | 1.8 ± 0.55 | 3.0 × 10-6 | 1.0 × 106 | |
G | 1.0 (± 0.12) × 105 | 0.051 ± 0.014 | 5.1 × 10-7 | 6.1 × 106 | |
A | 1.6 (± 0.032) × 105 | 0.18 ± 0.0069 | 1.1 × 10-6 | 2.8 × 106 | |
G | C | 44 ± 7.2 | 55 ± 3.8 | 1.3 | - |
U3 | 230 | 0.002 | 8.7 × 10-6 | 1.4 × 105 | |
A3 | 1.0 × 105 | 0.015 | 1.5 × 10-7 | 8.3 × 106 | |
G | 1.0 (± 0.0071) × 105 | 0.0029 (± 8.6) × 10-4 | 2.9 × 10-8 | 4.3 × 107 |