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Table 4 Pre-steady state fidelity

From: Biochemical characterization of the fidelity of poliovirus RNA-dependent RNA polymerase

Sym-Sub NTP K d (μM) k pol (sec-1) k pol /K d (μM -1 sec-1) Fidelity1
U A 120 ± 74 74 ± 22 0.62 -
  G2 1100 ± 100 0.004 (± 3.2) × 10-4 3.6 × 10-6 1.7 × 105
  U 1.7 (± 0.53) × 105 0.14 ± 0.053 8.2 × 10-7 7.5 × 105
  C3 3000 5.4 × 10-4 1.8 × 10-7 3.4 × 106
C G 1.6 ± 2.3 15 ± 9.2 9.4 -
  A 1.0 × 104 7.9 7.9 × 10-4 1.2 × 104
  U 270 ± 120 0.0079 ± 0.0014 2.9 × 10-5 3.2 × 105
  C3 620 0.0017 2.7 × 10-6 3.4 × 106
A U 27 ± 0.95 84 ± 0.48 3.1 -
  C 6.0 (± 0.70) × 105 1.8 ± 0.55 3.0 × 10-6 1.0 × 106
  G 1.0 (± 0.12) × 105 0.051 ± 0.014 5.1 × 10-7 6.1 × 106
  A 1.6 (± 0.032) × 105 0.18 ± 0.0069 1.1 × 10-6 2.8 × 106
G C 44 ± 7.2 55 ± 3.8 1.3 -
  U3 230 0.002 8.7 × 10-6 1.4 × 105
  A3 1.0 × 105 0.015 1.5 × 10-7 8.3 × 106
  G 1.0 (± 0.0071) × 105 0.0029 (± 8.6) × 10-4 2.9 × 10-8 4.3 × 107
  1. 1(k pol /K d )c + (k pol /K d )i/(k pol /K d )i. k pol and K d values from Table IV.
  2. 2The last two rows (for each sym/sub) represent transversion mutations.
  3. 3Kinetic values for which no error is reported were derived from only two NTP concentrations (see Table III).