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Table 4 Pre-steady state fidelity

From: Biochemical characterization of the fidelity of poliovirus RNA-dependent RNA polymerase

Sym-Sub

NTP

K d (μM)

k pol (sec-1)

k pol /K d (μM -1 sec-1)

Fidelity1

U

A

120 ± 74

74 ± 22

0.62

-

 

G2

1100 ± 100

0.004 (± 3.2) × 10-4

3.6 × 10-6

1.7 × 105

 

U

1.7 (± 0.53) × 105

0.14 ± 0.053

8.2 × 10-7

7.5 × 105

 

C3

3000

5.4 × 10-4

1.8 × 10-7

3.4 × 106

C

G

1.6 ± 2.3

15 ± 9.2

9.4

-

 

A

1.0 × 104

7.9

7.9 × 10-4

1.2 × 104

 

U

270 ± 120

0.0079 ± 0.0014

2.9 × 10-5

3.2 × 105

 

C3

620

0.0017

2.7 × 10-6

3.4 × 106

A

U

27 ± 0.95

84 ± 0.48

3.1

-

 

C

6.0 (± 0.70) × 105

1.8 ± 0.55

3.0 × 10-6

1.0 × 106

 

G

1.0 (± 0.12) × 105

0.051 ± 0.014

5.1 × 10-7

6.1 × 106

 

A

1.6 (± 0.032) × 105

0.18 ± 0.0069

1.1 × 10-6

2.8 × 106

G

C

44 ± 7.2

55 ± 3.8

1.3

-

 

U3

230

0.002

8.7 × 10-6

1.4 × 105

 

A3

1.0 × 105

0.015

1.5 × 10-7

8.3 × 106

 

G

1.0 (± 0.0071) × 105

0.0029 (± 8.6) × 10-4

2.9 × 10-8

4.3 × 107

  1. 1(k pol /K d )c + (k pol /K d )i/(k pol /K d )i. k pol and K d values from Table IV.
  2. 2The last two rows (for each sym/sub) represent transversion mutations.
  3. 3Kinetic values for which no error is reported were derived from only two NTP concentrations (see Table III).