Table 3
From: Biochemical characterization of the fidelity of poliovirus RNA-dependent RNA polymerase
Sym-Sub | NTP | [NTP] (μM) | Amplitude (nM) | kobs(sec-1) |
|---|---|---|---|---|
U | G1,2 | 375 | 350 (± 24) | 1.0 (± 0.26) × 10-3 |
750 | 200 (± 58) | 1.5 (± 0.62) × 10-3 | ||
1500 | 300 (± 24) | 2.2 (± 0.54) × 10-3 | ||
U1,2 | 750 | 640 (± 54) | 3.5 (± 0.69) × 10-4 | |
1500 | 490 (± 44) | 8.9 (± 0.29) × 10-4 | ||
3000 | 380 (± 14) | 2.8 (± 0.27) × 10-3 | ||
C | 1500 | 180 (± 35) | 1.8 (± 0.64) × 10-4 | |
3000 | 76 (± 13) | 2.7 (± 1.2) × 10-4 | ||
C | A | 5 | 47 (± 14) | 2.4 (± 1.1) × 10-3 |
10 | 85 (± 16) | 8.30 (± 0.86) × 10-3 | ||
U1,2 | 100 | 34 (± 4.7) | 2.30 (± 0.82) × 10-3 | |
200 | 79 (± 28) | 2.80 (± 1.7) × 10-3 | ||
750 | 82 (± 6.3) | 6.50 (± 1.6) × 10-3 | ||
1500 | 53 (± 7.2) | 6.2 (± 2.0) × 10-3 | ||
C3 | 750 | 470 | 9.3 × 10-4 | |
1500 | 640 | 1.2 × 10-3 | ||
A | C1 | 750 | 340 (± 26) | 1.3 (± 0.21) × 10-3 |
1500 | 250 (± 17) | 5.1 (± 2.3) × 10-3 | ||
G | 750 | 270 (± 100) | 1.8 (± 0.74) × 10-4 | |
1500 | 110 | 8.5 × 10-4 | ||
A3 | 750 | 5 | 1.6 × 10-3 | |
1500 | 23 (± 5.2) | 1.7 (± 1.0) × 10-3 | ||
G | U | 750 | 190 (± 6.9) | 1.3 (± 1.0) 10-3 |
1500 | 280 (± 26) | 8.3 (± 1.4) × 10-4 | ||
A1,3 | 750 | 25 | 2.5 × 10-5 | |
1500 | 18 | 2.1 × 10-5 | ||
G3 | 750 | 520 | 3.4 × 10-5 | |
1500 | 540 | 3.6 × 10-5 |