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Table 3 Table 3

From: Biochemical characterization of the fidelity of poliovirus RNA-dependent RNA polymerase

Sym-Sub NTP [NTP] (μM) Amplitude (nM) kobs(sec-1)
U G1,2 375 350 (± 24) 1.0 (± 0.26) × 10-3
   750 200 (± 58) 1.5 (± 0.62) × 10-3
   1500 300 (± 24) 2.2 (± 0.54) × 10-3
  U1,2 750 640 (± 54) 3.5 (± 0.69) × 10-4
   1500 490 (± 44) 8.9 (± 0.29) × 10-4
   3000 380 (± 14) 2.8 (± 0.27) × 10-3
  C 1500 180 (± 35) 1.8 (± 0.64) × 10-4
   3000 76 (± 13) 2.7 (± 1.2) × 10-4
C A 5 47 (± 14) 2.4 (± 1.1) × 10-3
   10 85 (± 16) 8.30 (± 0.86) × 10-3
  U1,2 100 34 (± 4.7) 2.30 (± 0.82) × 10-3
   200 79 (± 28) 2.80 (± 1.7) × 10-3
   750 82 (± 6.3) 6.50 (± 1.6) × 10-3
   1500 53 (± 7.2) 6.2 (± 2.0) × 10-3
  C3 750 470 9.3 × 10-4
   1500 640 1.2 × 10-3
A C1 750 340 (± 26) 1.3 (± 0.21) × 10-3
   1500 250 (± 17) 5.1 (± 2.3) × 10-3
  G 750 270 (± 100) 1.8 (± 0.74) × 10-4
   1500 110 8.5 × 10-4
  A3 750 5 1.6 × 10-3
   1500 23 (± 5.2) 1.7 (± 1.0) × 10-3
G U 750 190 (± 6.9) 1.3 (± 1.0) 10-3
   1500 280 (± 26) 8.3 (± 1.4) × 10-4
  A1,3 750 25 2.5 × 10-5
   1500 18 2.1 × 10-5
  G3 750 520 3.4 × 10-5
   1500 540 3.6 × 10-5
  1. 1Higher molecular weight products (12-, 13- and/or 14 mers) were observed, but data not included to estimate single turnovers.
  2. 2Data over 500 nM omitted.
  3. 3So few data points were available that there was no standard error for the single exponential calculations. In some cases, incorporation was so inefficient that data did not resemble a single exponential. Therefore, zero and the highest datapoint were used, yielding no standard error.