From: Biochemical characterization of the fidelity of poliovirus RNA-dependent RNA polymerase
NTP | Kd(uM) | Factor* | kpol(sec-1) | Factor* |
---|---|---|---|---|
ATP | 1.2 (± 0.74) × 102 | 74 (± 22) | ||
UTP-untrapped** | 1.7 (± 0.53) × 105 | 0.14 (± 0.052) | ||
UTP-heparin trap*** | 5.3 (± 3.0) × 104 | -3.2 | 0.032 (± 0.02) | -4.4 |
UTP-cold trap*** | 5.3 (± 0.065) × 104 | -3.2 | 0.027 (± 4.3 × 10-4) | -5.2 |