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Table 2

From: Biochemical characterization of the fidelity of poliovirus RNA-dependent RNA polymerase

NTP

Kd(uM)

Factor*

kpol(sec-1)

Factor*

ATP

1.2 (± 0.74) × 102

 

74 (± 22)

 

UTP-untrapped**

1.7 (± 0.53) × 105

 

0.14 (± 0.052)

 

UTP-heparin trap***

5.3 (± 3.0) × 104

-3.2

0.032 (± 0.02)

-4.4

UTP-cold trap***

5.3 (± 0.065) × 104

-3.2

0.027 (± 4.3 × 10-4)

-5.2

  1. *Factor by which data with trap differ from data without trap.
  2. **Only 12 mers were analyzed (because no 11 mers were visible). Data over 500 nM were disregarded.
  3. ***All data.