From: Biochemical characterization of the fidelity of poliovirus RNA-dependent RNA polymerase
Sym-Sub U | ||
---|---|---|
[ATP] (μM) | Amplitude (nM) | kobs (sec-1) |
50 | 110 (± 4.4) | 24 (± 4.7) |
100 | 160 (± 25) | 30 (± 21) |
200 | 100 (± 6.1) | 47 (± 16) |
K d ( μM) | k pol (sec-1) | kpol/Kd ( μM-1 sec-1) |
120 (± 74) | 74 (± 33) | 0.62 |
Sym-Sub C | ||
[GTP] ( μM) | Amplitude (nM) | kobs (sec-1) |
1 | 150 (± 13) | 6.7 (± 1.1) |
2 | 120 (± 4.7) | 9.7 (± 1.3) |
3 | 120 (± 13) | 9.9 (± 3.4) |
K d ( μM) | k pol (sec-1) | k pol / Kd ( μM-1 sec-1) |
0.93 (± 0.45) | 13 (± 2.0) | 9.4 |
Sym-Sub A | ||
[UTP] ( μM) | Amplitude (nM) | kobs (sec-1) |
75 | 270 (± 13) | 62 (± 17) |
150 | 260 (± 6.7) | 71 (± 25) |
500 | 250 (± 71) | 80 (± 21) |
K d ( μM) | k pol (sec-1) | k pol / Kd ( μM-1 sec-1) |
27 (± 0.95) | 84 (± 0.48) | 3.1 |
Sym-Sub G | ||
[CTP] ( μM) | Amplitude (nM) | kobs (sec-1) |
10 | 89 (± 16) | 10 (± 4.8) |
25 | 100 (± 36) | 19 (± 2.1) |
100 | 110 (± 38) | 38 (± 5.8) |
K d ( μM) | k pol (sec-1) | k pol / Kd ( μM-1 sec-1) |
44 (± 7.2) | 55 (± 3.8) | 1.25 |