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Table 1 Table 1

From: Biochemical characterization of the fidelity of poliovirus RNA-dependent RNA polymerase

Sym-Sub U   
[ATP] (μM) Amplitude (nM) kobs (sec-1)
50 110 (± 4.4) 24 (± 4.7)
100 160 (± 25) 30 (± 21)
200 100 (± 6.1) 47 (± 16)
K d ( μM) k pol (sec-1) kpol/Kd ( μM-1 sec-1)
120 (± 74) 74 (± 33) 0.62
Sym-Sub C   
[GTP] ( μM) Amplitude (nM) kobs (sec-1)
1 150 (± 13) 6.7 (± 1.1)
2 120 (± 4.7) 9.7 (± 1.3)
3 120 (± 13) 9.9 (± 3.4)
K d ( μM) k pol (sec-1) k pol / Kd ( μM-1 sec-1)
0.93 (± 0.45) 13 (± 2.0) 9.4
Sym-Sub A   
[UTP] ( μM) Amplitude (nM) kobs (sec-1)
75 270 (± 13) 62 (± 17)
150 260 (± 6.7) 71 (± 25)
500 250 (± 71) 80 (± 21)
K d ( μM) k pol (sec-1) k pol / Kd ( μM-1 sec-1)
27 (± 0.95) 84 (± 0.48) 3.1
Sym-Sub G   
[CTP] ( μM) Amplitude (nM) kobs (sec-1)
10 89 (± 16) 10 (± 4.8)
25 100 (± 36) 19 (± 2.1)
100 110 (± 38) 38 (± 5.8)
K d ( μM) k pol (sec-1) k pol / Kd ( μM-1 sec-1)
44 (± 7.2) 55 (± 3.8) 1.25