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Table 3 Comparison of HA titer of influenza and baculoviruses with hemadsorption to insect and mammalian cells expressing HA.

From: Receptor binding specificity of recent human H3N2 influenza viruses

 

Hemagglutination titer

Hemadsorption (A540)1

 

Influenza virus

Recombinant baculovirus2

MDCK cells

Sf9-cells

 

Human

Chicken

Human

Chicken

Human

Chicken

Human

Chicken

OK/323/03

64

0

512

0

0.73 ± 0.07

0.03 ± 0.005

0.71 ± 0.02

0.26 ± 0.06

OK/369/05 wt

64

0

128

0

0.76 ± 0.02

0.20 ± 0.005

0.80 ± 0.09

0.26 ± 0.03

OK/369/05 S138A

N/A3

N/A

64

0

N/A

N/A

0.82 ± 0.07

0.31 ± 0.08

OK/372/05

64

32

64

0

0.74 ± 0.004

0.40 ± 0.01

1.05 ± 0.09

0.35 ± 0.06

  1. 1 Mean of 4 experiments ± standard deviation
  2. 2 Baculovirus was concentrated 10-fold before HA titration
  3. 3 N/A = not applicable
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Virology Journal

ISSN: 1743-422X