Figure 6

Distribution of diversifying capsid residues relative to functional domains. Diversifying residues in the HRV2 capsid pentamer (Verdauger et al., 2000) overlaid onto the characterized HRV antigenic sites (Appleyard et al., 1990; Hastings et al., 1990; Speller et al., 1993; Hewat and Blaas, 1996; Hewat et al., 1998). B. Diversifying residues in the HRV16 capsid pentamer (Hadfield, et al., 1997) overlaid onto the characterized ICAM1 cellular receptor contacts (Bella et al., 1999). C. Diversifying residues in the HRV2 capsid pentamer (Verdauger et al., 2000) overlaid onto the characterized LDLR cellular receptor contacts (Verdauger et al., 2004). Diversifying residues are shown in red, shaded according to corresponding dN/dS values as indicated by the scale bar below panel C; green, antigenic residues (A); ICAM1 receptor contacts (B), and LDLR contacts (C); yellow, diversifying residues that directly overlap functional residues. Inset histogram, distribution of minimal distances between α-carbons of diversifying residues and antigenic sites (A), ICAM1 contact residues (B), and LDLR contact residues (C); Y-axis is simple frequency count, with a range that varies for each panel; p values provide frequency at which an average minimum distance similar to that for the observed distribution was detected when the locations of the diversifying residues were randomized on each pentamer surface, and minimal distances to antigenic site residues (A), ICAM1R contact residues (B), and LDLR contact residues (C) were measured (n = 100,000 randomizations).