Figure 3
Potential interaction between the anticodon region of tRNAHis and HIV-1 proviral genomes with mutated A-loop regions. The A-loop PBS region of NL4-His-HisAC depicting the nucleotides displayed on the loop of an RNA stem loop that are complementary to the anticodon of tRNAHis. In the case of NL4-His-HisAC, complete complementarity is noted consisting of eight consecutive nucleotides. Viruses in which the PBS complementary to tRNAHis were unstable following in vitro replication included those derived from NL4-His and NL4-His-SerAC (bolded, unstable). Interactions between the anticodon of tRNAHis and NL4-His or NL4-His-SerAC consisted of two potential base pair interactions (shaded). In contrast, interaction between the anticodon of tRNAHis and viruses which stably maintained a PBS complementary to tRNAHis (NL4-His-IleAC, NL4-His-MetAC or NL4-His-ThrAC) revealed three potential base pair interactions (shaded). The virus NL4-GlnAC was also stable following in vitro replication. A three-nucleotide interaction could be postulated (in shaded regions) consist of two of the three nucleotides in the anticodon of tRNAHis. This putative interaction is denoted with a question mark.