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Figure 2 | Virology Journal

Figure 2

From: Importance of A-loop complementarity with tRNAHis anticodon for continued selection of tRNAHis as the HIV reverse transcription primer

Figure 2

Replication of HIV-1 with wild type and altered U5-PBS in SupT1 cells. Panel A. Replication of HIV (NL4-WT) and HIV which contain U5-PBS complementary to tRNAHis. Infections were initiated with equal amounts of infectious virus as determined by the JC53-BL assay. Virus replication in SupT1 cells was monitored over 42 days of culture. The amount of virus produced was analyzed by p24 ELISA. The identity of the viruses are as marked. Panel B. Comparison of the replication of the wild type virus with mutant viruses containing PBS complementary to tRNAHis with different A-loop regions. The identity of the viruses are as marked. Panel C. Comparison of the replication of the wild type virus with HIV-1 genomes with the PBS complementary to tRNAHis with different A-loop regions. The identity of the viruses are as marked.

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