Beet necrotic yellow vein virus accumulates inside resting spores and zoosporangia of its vector Polymyxa betae BNYVV infects P. betae

Lubicz, Jeanmarie Verchot; Rush, Charles M; Payton, Mark; Colberg, Terry

doi:10.1186/1743-422X-4-37

Virology Journal

Table 3 Distribution of immunogold label in virus infected and healthy P. betae zoospores

From: Beet necrotic yellow vein virus accumulates inside resting spores and zoosporangia of its vector Polymyxa betae BNYVV infects P. betae

Antisera	P. betae Samples^a	No. Fields (1 μm²)	Cytoplasm^b	Storage Body	Vacuoles/Vesicles^c	Nucleus	Flagella	Between Zoospores^d	Zoosporangial Wall
Replicase	Infected	50	7.35 ± 1.68o	2.40 ± 0.67o	6.19 ± 1.21o	1.33 ± 0.33	1.56 ± 1.18	4.11 ± 1.60o	9.04 ± 2.14o
	Healthy	7	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	ND	0.00 ± 0.00	0.20 ± 0.20	0.00 ± 0.00
Coat	Infected	98	1.17+0.22	0.21+0.08	2.57+0.42o	0.73+0.37	0.32+0.19	1.00+0.25	1.00+0.77
	Healthy	ND	ND	ND	ND	ND	ND	ND	ND
RTD	Infected	22	2.14 ± 0.59o	0.24 ± 0.18	3.10 ± 0.97o	1.00 ± 0.58	0.54 ± 1.21	0.50 ± 0.32	ND
	Healthy	ND	ND	ND	ND	ND	ND	ND	ND
P42	Infected	95	5.30 ± 1.51o	1.32 ± 0.33o	5.03 ± 0.95o	0.81 ± 0.29	0.19 ± 0.11	7.05 ± 0.77o	1.00 ± 0.71
	Healthy	18	0.07 ± 0.26	0.00 ± 0.00	0.40 ± 0.74	0.00 ± 0.52	0.33 ± 0.00	0.50 ± 0.82	0.60 ± 1.34
P13	Infected	111	4.38 ± 0.81o	0.60 ± 0.28	2.38 ± 0.67o	0.44 ± 0.29	1.38 ± 0.50	2.13 ± 0.39	2.33 ± 0.80
	Healthy	64	0.00 ± 1.08	0.07 ± 0.34	0.39 ± 0.89	0.53 ± 0.80	0.00 ± 0.00	0.81 ± 1.27	0.00 ± 0.00
P15	Infected	34	0.22 ± 0.10	0.40 ± 0.20	0.70 ± 0.21	1.67 ± 0.54	1.00 ± 0.44	0.71 ± 0.29	0.63 ± 0.22
	Healthy	24	0.26 ± 0.14	0.55 ± 0.15	1.24 ± 0.38	0.00 ± 0.00	0.18 ± 0.18	0.00 ± 0.25	0.75 ± 0.00
P14	Infected	119	1.29 ± 0.19	0.46 ± 0.10	0.98 ± 0.17	0.75 ± 0.37	0.27 ± 0.08	1.28 ± 0.17	1.48 ± 0.38
	Healthy	60	0.05 ± 0.03	0.06 ± 0.03	0.49 ± 0.12	0.00 ± 0.00	0.00 ± 0.00	0.58 ± 0.14	0.22 ± 0.15
P25	Infected	105	2.83 ± 0.33o	1.55 ± 0.34o	2.92 ± 0.39o	2.88 ± 1.17o	0.91 ± 0.39	1.93 ± 0.34	1.67 ± 1.31o
	Healthy	9	0.10 ± 0.10	0.00 ± 0.00	0.80 ± 0.44	0.00 ± 0.00	0.00 ± 0.00	ND	0.60 ± 0.24
P31	Infected	56	3.48+0.59o	1.30+0.31o	4.10+0.54o	3.40+1.68o	3.38+0.77o	3.96+0.44o	4.00+1.14o
	Healthy	75	0.44 ± 0.10	0.08 ± 0.05	0.26 ± 0.08	0.27 ± 0.13	0.40 ± 0.31	0.91 ± 0.12	0.09 ± 0.09
Buffer	Infected	110	0.27 ± 0.14	0.02 ± 0.02	0.38 ± 0.13	0.13 ± 0.10	0.10 ± 0.06	0.85 ± 0.18	0.13 ± 0.13
	Healthy	75	0.17 ± 0.10	0.00 ± 0.00	0.09 ± 0.07	0.17 ± 0.08	0.00 ± 0.00	0.00 ± 0.08	0.22 ± 0.00

^a Pairwise comparisons of each antiserum treatment with buffer or healthy samples, for each location was carried out using ANOVA methods as in Table 1. Means and standard errors are reported. Values with a o symbol identify means that differ significantly from the buffer control at p < 0.05. Values indicated in bold are virus infected and virus-free (healthy) samples that differ significantly, p < 0.05.
^bP. betae zoosporangia were identified in virus infected and healthy plants. These were isolated and compared for their reactivity with the various BNYVV antiserum.
^c Cytoplasm refers to the body of the zoospores.
^d Vacuoles and pinocytotic vesicles are grouped together. Pinocytotic vesicles seemed to form horseshoe or doughnut shaped bodies surrounding zoospore cytoplasm. Vacuoles are round or irregularly shaped vesicles that have no contents or fibrillar material.

Back to article page

ISSN: 1743-422X

Contact us

Submission enquiries: journalsubmissions@springernature.com