Beet necrotic yellow vein virus accumulates inside resting spores and zoosporangia of its vector Polymyxa betae BNYVV infects P. betae

Table 2 Distribution of immunogold label in virus infected P. betae resting spores^a

Antisera	No. Fields (1 μm²)	Wall Pb1-4^b	Pb5^b	Matrix^c	Storage Bodies	Vacuole	Central Body	Between Spores
Replicase	40	6.52 ± 1.69	6.29 ± 1.23	6.90 ± 2.20	12.38 ± 3.32	ND	11.04 ± 2.45	3.45 ± 1.65
Coat	35	1.16 ± 0.24	4.92 ± 1.93	1.53 ± 0.74	1.55 ± 0.49	2.46 ± 0.74	1.52 ± 0.36	1.90 ± 0.66
RTD	114	2.76 ± 0.32	2.12 ± 0.61	0.94 ± 0.36	2.65 ± 0.57	2.51 ± 0.40	2.14 ± 0.55	1.21 ± 0.28
P42	49	0.91 ± 0.15	0.88 ± 0.37	0.67 ± 0.36	1.32 ± 0.33	1.23 ± 0.48	1.08 ± 0.36	0.67 ± 0.25
P13	73	4.55 ± 0.75	2.12 ± 0.61	2.31 ± 0.51	1.98 ± 0.56	1.00 ± 0.39	1.61 ± 0.57	3.05 ± 1.17
P15	34	3.48 ± 0.55	1.27 ± 0.54	1.33 ± 0.50	3.55 ± 0.68	0.14 ± 0.14	3.67 ± 0.91	0.40 ± 0.31
P14	47	2.23 ± 0.29	2.70 ± 1.08	0.38 ± 0.16	0.43 ± 0.12	1.25 ± 0.52	0.80 ± 0.39	1.07 ± 0.22
P25	78	4.48 ± 0.42	3.07 ± 0.90	2.29 ± 0.49	2.62 ± 0.53	0.67 ± 0.33	2.58 ± 0.55	1.27 ± 0.39
P31	59	4.28 ± 0.48	2.43 ± 0.72	0.70 ± 0.22	0.68 ± 0.20	1.67 ± 0.66	1.42 ± 0.34	3.71 ± 0.96
Buffer	54	0.38 ± 0.22	0.00 ± 0.00	0.19 ± 0.10	0.40 ± 0.18	0.27 ± 0.23	0.10 ± 0.07	0.08 ± 0.08

^a All data were analyzed using PC SAS Version 9 (SAS Institute, Cary, NC). Analysis of variance (ANOVA) models were created to account for variability attributed to subcellular location and antisera for the total number of fields. Pairwise comparison of each antiserum treatment with buffer, for each location was carried out using ANOVA methods. Means and standard errors are reported. Values in bold identify means that differ significantly from the buffer control at p < 0.05.
^bP. betae resting spores have 5 wall layers (Pb1-5). Data for Pb5 was scored separately from Pb1-4. This is because Pb5 is thicker and distinct from layers Pb1-4. It is also suggested to have a role in spore germination, making it functionally distinct from the other wall layers. Because Pb5 might be functionally distinct from the other wall layers we decided to score Pb5 separately from Pb1-4.
^c The matrix is the cytoplasmic layer between the wall and the central body of the resting spore. It stains light grey and often forms conical projections extending beyond Pb5. Its function is unknown.

ISSN: 1743-422X