Figure 2
From: Characterization of gene expression on genomic segment 7 of infectious salmon anaemia virus
Autoradiograph of SDS-PAGE of expressed proteins labeled with [35S]methionine in a coupled in vitro TNT System with the different constructs in pCR®II-TOPO® plasmid and in TO cells infected with ISAV as in Fig. 1, immunoprecipitated with rabbit antiserum to purified ISAV. The prestained protein standards (Invitrogen Life Sciences) are drawn in on the left while corresponding molecular masses of immunoprecipitated ISAV proteins are indicated on the right. (A) Lanes 1 to 8 contain radiolabeled proteins synthesized in uninfected TO cells, ISAV-infected TO cells at 24, 48 and 96 hr post-infection, and in TNT System with constructs of ORFs in ISAV segments 3–6, respectively. (B) Lanes 1 to 8 contain radiolabeled proteins synthesized in uninfected TO cells, ISAV-infected TO cells at 24, 48 and 96 hr post-infection, and in TNT System with constructs of ISAV segment 7 ORFs 1 and 2 and segment 8 ORFs 1 and 2, respectively.