Figure 2
From: Transplacental murine cytomegalovirus infection in the brain of SCID mice
Congenital MCMV infection in E18 fetal membranes. Pregnant SCID mice were injected with 103 PFU of MCMV at E4. A: H&E staining of an E18 SCID mouse placenta with extraembryonic membranes. ST, spongiotrophoblastic zone; LT, labyrinthine zone; visceral yolk sac in box. B: Anti-MCMV immunostaining (green; arrow) for viral antigens in the mesodermal layer of the visceral yolk sac boxed in (A). C: In situ hybridization (purple; arrow) for MCMV RNA in the mesodermal layer of the visceral yolk sac boxed in (A). D: Anti-MCMV immunostaining (green) in endothelial cells (anti-CD31+: red) in the walls of blood vessels (arrows) within the visceral yolk sac of an E18 embryo. E: Anti-MCMV immunostaining (green, arrows) within macrophages (anti-F4/80: red) in the visceral yolk sac mesoderm of an E18 embryo. A,D&E: Bisbenzimide nuclear stain (blue). A: Bar = 1 mm; B&C: Bars = 100 μm, D&E: Bars = 20 μm.