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Table 4 Replication of rDEN1/4(ME) chimeric viruses in Aedes aegypti

From: Vaccine candidates for dengue virus type 1 (DEN1) generated by replacement of the structural genes of rDEN4 and rDEN4Δ30 with those of DEN1

Virus1

No. mosquitoes

Body2

Head2

% Dissemination3

  

% infected

Mean virus titer (log10 PFU/body)

% infected

Mean virus titer (log10 PFU/head)

 

DEN1

24

88

4.5 ± 0.1

88

4.7 ± 0.1

100

DEN4

24

88

3.0 ± 0.2

71

2.7 ± 0.3

81

rDEN1/4(ME)

24

63

2.4 ± 0.3

42

3.0 ± 0.4

67

rDEN1/4Δ30(ME)

24

0

< 0.4

0

< 0.4

0

  1. 1 Assuming a 2 ul bloodmeal, 4.3 log10 PFU virus of each virus were ingested.
  2. 2 Presence of virus in mosquito homogenates was determined by plaque assay in Vero cells. Mean virus titer was determined using values only from samples with detectable virus.
  3. 3 Calculated as no. infected heads/no. infected bodies.