Figure 2

fAPN expression rescues FIPV-1146 and TGEV infection in non-permissive BHK-21 cells. A) CRFK, BHK, or BHK cells transfected with fAPN/pcDNA3.1D/TOPO were separately infected with FIPV-1146 for 9 h. Monolayers were stained with 17B71 monoclonal antibody to detect FIPV-1146 infections. B) A72, BHK, or BHK cells transfected with fAPN/pcDNA3.1D/TOPO were separated infected with TGEV for 8 h. Monolayers were labeled with rabbit polyclonal antibody 367 to detect TGEV infections. C) BHK and BHK transfected with fAPN/pcDNA3.1D/TOPO cells were separately infected with FIPV-1146 or TGEV. Viral infectivity was determined by counting >300 cells in three separate experiments. Error bars represent the standard deviation from the mean.