Figure 6

Viral capture assay. HIV-1 and HSV-2 capture by antibodies targeted against cell surface CD44 antigen or HSV-2 gB or gD in HIV-1- and HSV-2 dually infected P4P cells (A), CEM cells (B) and HIV-1-infected P4P cells transfected by gB or gD (C). Levels of captured virus were determined by HIV-1 RNA (open square) and HSV-2 DNA (bold square) quantification by real time PCR and compared to capture by a negative control antibody (biotin labeled anti-mouse) to derive fold-over background values. Data are representative of three independent experiments.