Table 1 Primers and conditions for amplification.
Primer Set | Sense 5' – 3' | Antisense 5' – 3' | Size, (pb) |
|---|---|---|---|
HPV16 5' region | caccacctcatctacctc | caggatgtagcaaatatag | 292 |
HPV16 Enhancer | ctatatttgctacatcctg | cagcggtatgtaaggc | 319 |
HPV16 3' region | gccttacataccgctg | gctctgtgcataactgtgg | 382 |
HPV18 5' region | cgtgccaggaagtaatatg | gatgctgtaaggtgtgcag | 301 |
HPV18 Enhancer | ctgcacaccttacagcatc | gggtagacagaatgttgg | 368 |
HPV18 3' region | ccaacattctgtctaccc | gttccgtgcacagatcag | 266 |
HPV16 E6-E7 | gggaatccatatgctgtatg | gggaagcttttatggtttctgagaacag | 597 |
HPV18 E6-E7 | gaatttgcattcaaagatttatttg | gggggaattcttactgctgggatgcacacc | 673 |
p53 | ctgaggttggctctgactgtaccaccatcc | ctcattcagctctcggaacatctcgaagcg | 390 |