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Figure 2 | Virology Journal

Figure 2

From: The effects of DNA methylation and histone deacetylase inhibitors on human papillomavirus early gene expression in cervical cancer, an in vitro and clinical study

Figure 2

Expression of E6/E7, methylation at LCR and ChIP assays. 2a. E6/E7 expression was assayed by RT-PCR. CaLo cells no changes in expression. In HeLa, hydralazine and hydralazine/valproate up-regulated the transcript 1.17- and 2.38-fold, respectively. In SiHa cells only the combination of hydralazine/valproate led to a 0.5-fold increase, while in CasKi, hydralazine, valproate, and both increased expression by 0.52-, 0.67-, and 0.83-fold, respectively. 2b. Hydralazine/valproate led to a small increase in the intensity of the bands after digestion with the enzyme McrBC in the three LCR regions analyzed as compared with untreated cells, indicating a certain degree of hypomethylation. 3c. ChIP assays with anti acetylated H4. In the CaLo, acetylated H4 was detected in the three regions analyzed; hydralazine, valproate, and both decreased the enrichment for acetylated H4. In line with increased E6/E7 expression in SiHa had an enrichment of acetylated H4 at the 5' region of the LCR, whereas only valproate affected this at the enhancer region.

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Virology Journal

ISSN: 1743-422X