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Figure 2 | Virology Journal

Figure 2

From: Differential inhibition of human cytomegalovirus (HCMV) by toll-like receptor ligands mediated by interferon-beta in human foreskin fibroblasts and cervical tissue

Figure 2

The effect of time of TLR stimulation on HCMV infection and IL-8 secretion in Foreskin Fibroblasts. A. Cell monlayers were treated with medium alone, Poly I:C, LPS or CpG 2395 (all at 10 μg/ml) for either 2 hours, 24 hours, 48 hours, or for 24 hours followed by a period of 24 hours with fresh complete medium (24/24 group) and then challenged with CMVPT30-gfp. At day 10 post-infection the number of gfp-expressing foci were determined by fluorescence microscopy and the percent inhibition was calculated based on medium control-treated cells. B. Cells were stimulated with medium alone (control, C) or the indicated dose of the TLR ligands for a period of 2 hours, 24 hours, 48 hours, or for 24 hours followed by a period of 24 hours with fresh complete medium (24/24 group). Culture supernatants were immediately harvested and IL-8 levels determined by ELISA. For both A and B bars represent mean ± SD of triplicate cultures. * indicates P ≤ 0.05 compared to control. ** indicates P ≤ 0.001 compared to control.

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Virology Journal

ISSN: 1743-422X