Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses

Table 2 Results of GeNorm analysis

Virus	Cell	B2M	PP1A	EEF1G	SDHA	GAPDH	HMBS	18sRNA	PGK1	B-ACT	TBP	sum_RGC
HIV-1_BaL	MDDC	0.98	0.78	1.04	1.26	0.83	0.84	0.98	1.09	1.45	0.97	*10.22*
HIV-1_NL4.3	HELA	0.84	0.53	0.75	0.88	0.60	0.84	0.93	0.78	0.90	0.72	*7.77*
HIV-1_NL4.3	HUT78	1.11	1.18	1.34	0.96	1.35	1.13	2.20	1.20	1.38	1.05	*12.90*
HIV-1_RFW	HUT78	1.21	0.64	0.83	0.90	0.99	1.17	1.53	0.87	0.97	1.16	*10.27*
HSV-1_NC1	HELA	0.80	0.65	1.33	0.62	0.91	0.59	0.89	0.60	0.92	0.74	*8.05*
HSV-1_NC1	HEP2	0.41	0.42	0.44	0.50	0.37	0.38	0.60	0.41	0.57	0.54	*4.64*
VZV_Schenke	MDDC	0.99	0.93	0.96	1.09	0.93	1.23	1.83	1.30	1.02	0.94	*12.22*
VZV_Schenke	HFF	1.56	1.09	1.12	1.34	1.45	1.75	1.94	1.87	1.70	1.54	*15.36*
CMV_Towne	HFF	0.65	0.58	0.60	0.60	0.82	0.60	1.14	0.58	0.74	0.63	*6.8*
CMV_Toledo	HFF	0.86	0.61	0.75	0.76	0.71	0.90	1.22	0.73	0.86	0.77	*7.87*
	*sum* _v	*9.41*	*7.41*	*9.16*	*8.91*	*8.74*	*9.65*	*13.26*	*9.43*	*10.51*	*9.06*

The standard deviations of reference gene expression as determined by GeNorm are shown. Abbreviations: Sum_v: Sum of viral infection GeNorm values; sum_RGC: sum of reference gene GeNorm values

ISSN: 1743-422X