Effects of pre-transfection treatment of methylcellulose on expression of the PV L1 genes in mouse primary KCs. Newly isolated mouse KCs were suspended in semisolid KC medium containing different concentration of methylcellulose for 48 h. They were then transfected with the four PV L1 gene expression constructs and grown in KC-SF complete medium for 48 h before harvested for analysis of L1 gene expression. (A). L1 transcripts were assessed by quantitative RT-PCR. β-tubulin transcript was analysed as an internal control. Up panel: Representative electrophoresis of the L1 and tubulin mRNA qRT-PCR products. Lower panel: Results are shown with the means (± S.E.M) of duplicate transfection assays from two separate experiments. (B). Expression of the L1 proteins analysed by Western blot is representative of duplicate transfection from two separate experiments. β-tubulin (Tub) was used as comparable loading control.