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Figure 5 | Virology Journal

Figure 5

From: The internal initiation of translation in bovine viral diarrhea virus RNA depends on the presence of an RNA pseudoknot upstream of the initiation codon

Figure 5

Mutagenesis of stem 1 and stem 2 of the proposed pseudoknot structure in the BVDV SD-1 5' UTR. A. Schematic drawing of wild-type plasmids and pseudoknot mutants. Plasmid pSBCSSD1-9LUC (Fig. 4) was used as basic plasmid for construction of the pseudoknot mutants. Altered nucleotides are boxed. In mutants M1 and M2 stem 2 base-pairing is disturbed, in M4, M5, and M6 the stem 1 complementarity is impaired.

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