Figure 6

Confocal microscopy of gK cell-surface expression and endocytosis to the TGN mediated by selected UL20p mutants. Vero cells were co-transfected with gKD1V5 as well as with plasmids encoding wild-type or mutant UL20p, as with figures 4 and 5. Twenty-four hours post-transfection, cells were incubated under live conditions with aV5 (gK) mAb for 6 hours. Cells were washed thoroughly, fixed, and processed for confocal microscopy. After permeabilization, antibodies a3 × FLAG, aV5 and aTGN46 were used to identify, UL20p, gK and TGN46, respectively.