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Figure 4 | Virology Journal

Figure 4

From: The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment

Figure 4

The effect of UL20p amino terminal mutations on UL20p and gK colocalization in TGN cellular compartments. Vero cells were co-transfected with gK tagged with the V5 epitope (D1V5), as well as with plasmids encoding wild-type or different mutant UL20ps tagged with the 3 × FLAG epitope (UL20D1FLAG). Thirty-six hours post-transfection, cells were washed thoroughly, fixed, and processed for confocal microscopy. After permeabilization, rabbit anti-FLAG (α FLAG) mAb was used to detect UL20p, mouse anti-V5 (α V5) epitope was used to detect gK, and sheep aTGN46 mAb was used to identify the TGN. First three rows of the confocal pictures show co-localization of UL20p with gK, while rows 4–6 show colocalization of gK with TGN46.

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Virology Journal

ISSN: 1743-422X