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Figure 6 | Virology Journal

Figure 6

From: Roles of adjuvant and route of vaccination in antibody response and protection engendered by a synthetic matrix protein 2-based influenza A virus vaccine in the mouse

Figure 6

Relation between M2e(pep-nat)-specific Ab titer and protection against virus challenge. Cκ-positive M2e(pep-nat)-specific Ab titers were determined in pooled plasma (3–5 mice/group) collected 7–10 days before challenge of mice by localized nasal infection (5 μl X31, 1000 TCID50). Five days after challenge, virus titers were determined in nose, trachea and lung of individual mice and the group average was determined. The average reduction in virus titer on log10 basis compared to the control group (immunized with adjuvant alone) was taken as measure of strength of protection (y axis). A. Protection in nose (squares), trachea (triangles) and lung (circles) from nine groups of mice immunized by parenteral route is plotted against the M2e(pep-nat)-specific serum Ab titer (x axis). Non-linear regression analysis yielded sigmoidal regression curves with R2 of 0.79 for nose (stipulated) and lung (continuous) and of 0.65 for trachea (dashed). B. Serum Ab titers and protection in nose observed in mice immunized by the i.n. route (filled squares) are plotted together with the regression line and corresponding data points (open squares) from mice immunized by parenteral route (as in A). C. Serum Ab titers and protection in lung of mice immunized by i.n. route (filled circles) are plotted together with the regression line and corresponding data points (open circles) from mice after parenteral immunization (as in A).

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