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Table 1 E5 mutant M1 down-regulates surface expression of HLA-I to a lesser extent than the E5 protein does.

From: The E5 protein of the human papillomavirus type 16 down-regulates HLA-I surface expression in calnexin-expressing but not in calnexin-deficient cells

 

E5-GFP

p - value c

M1-GFP

 

E5-GFP

p - value c

M1-GFP

HLA-

57.77%

t- test

69.78%

KS-Db

0.29

t- test

0.20

surface

67.92%

0.009

75.67%

 

0.17

0.005

0.13

expressiona

75.67%

 

94.75%

 

0.15

 

0.04

 

69.16%

Wilcoxon

82.04%

 

0.20

Wilcoxon

0.11

 

59.35%

0.0313

82.78%

 

0.25

0.0313

0.10

 

62.08%

 

100.90%

 

0.23

 

0.02

Median

65.00%

 

82.41%

Median

0.215

 

0.105

Range

58%-76%

 

70%-100%

Range

0.17–0.29

 

0.02–0.2

  1. aValues of pEGFP-E5 and pEGFP-M1 were normalized to the values of the pEGFP-control (E5-GFP, M1-GFP), which was set as 100% expression of HLA-I surface expression.
  2. bKolmogorov-Smirnov (KS) test was performed with CellQuest™ software (BD Bioscience). This statistic defines the maximum vertical deviation between the two curves (pEGFP-E5 and pEGFP-control or pEGFP-M1 and pEGFP-control) as the statistic D. The p value of each single experiment was ≤ 0.001.
  3. cPaired two tailed paired student's t-test and Wilcoxon matched-pairs signed-ranks test values for the raw percentages of immunorreactive cells (left column) and for the D statistic (right column). The E5 mutant M1 does not affect HLA-I expression in the same extent than the original E5 protein does (p < 0.05 in all cases).