| E5-GFP |
p
- value
c
| M1-GFP | Â | E5-GFP |
p
- value
c
| M1-GFP |
HLA- | 57.77% |
t-
test
| 69.78% | KS-Db | 0.29 |
t-
test
| 0.20 |
surface | 67.92% |
0.009
| 75.67% | Â | 0.17 |
0.005
| 0.13 |
expressiona | 75.67% | Â | 94.75% | Â | 0.15 | Â | 0.04 |
 | 69.16% |
Wilcoxon
| 82.04% | Â | 0.20 |
Wilcoxon
| 0.11 |
 | 59.35% |
0.0313
| 82.78% | Â | 0.25 |
0.0313
| 0.10 |
 | 62.08% |  | 100.90% |  | 0.23 |  | 0.02 |
Median | 65.00% | Â | 82.41% | Median | 0.215 | Â | 0.105 |
Range | 58%-76% |  | 70%-100% | Range | 0.17–0.29 |  | 0.02–0.2 |
- aValues of pEGFP-E5 and pEGFP-M1 were normalized to the values of the pEGFP-control (E5-GFP, M1-GFP), which was set as 100% expression of HLA-I surface expression.
- bKolmogorov-Smirnov (KS) test was performed with CellQuest™ software (BD Bioscience). This statistic defines the maximum vertical deviation between the two curves (pEGFP-E5 and pEGFP-control or pEGFP-M1 and pEGFP-control) as the statistic D. The p value of each single experiment was ≤ 0.001.
- cPaired two tailed paired student's t-test and Wilcoxon matched-pairs signed-ranks test values for the raw percentages of immunorreactive cells (left column) and for the D statistic (right column). The E5 mutant M1 does not affect HLA-I expression in the same extent than the original E5 protein does (p < 0.05 in all cases).