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Figure 9 | Virology Journal

Figure 9

From: The E5 protein of the human papillomavirus type 16 down-regulates HLA-I surface expression in calnexin-expressing but not in calnexin-deficient cells

Figure 9

HPV16 E5 forms a ternary complex with calnexin and the HLA-I heavy chain. HeLa cells were transiently transfected with AU1-tagged codon-optimised HPV16 E5, M1, or empty vector. 24 h later CHAPS lysates were immunoprecipitated with antibodies against the E5-tag (anti-AU1). Precipitated immune complexes were separated by SDS-PAGE and Western blotted using anti-calnexin and anti-HLA-B, -C mAb (HC10), respectively (band marked with *). Molecular-mass markers in kDa are indicated at the left of the blots.

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Virology Journal

ISSN: 1743-422X