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Figure 3 | Virology Journal

Figure 3

From: The E5 protein of the human papillomavirus type 16 down-regulates HLA-I surface expression in calnexin-expressing but not in calnexin-deficient cells

Figure 3

Transporter activity of TAP is not influenced by HPV16 E5. Streptolysin Opermeabilized calnexin-proficient CEM-C7 and calnexin-deficient CEM-NKR cells (38) were analysed in a peptide translocation/glyosylation assay using the indicated input quantities of the radioiodinated reporter peptide TNKTRIDGQY (glycosylation consensus site underlined) in the presence or absence of ATP. The glycosylated fraction, indicative of TAP-mediated ER transport, is isolated by concanavalin A Sepharose and quantitated by γ-counting. No significant differences could be detected between HPV16 E5-expressing cells and the control cells irrespective from the presence (CEM-C7) or absence (CEM-NKR) of calnexin.

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Virology Journal

ISSN: 1743-422X