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Figure 2 | Virology Journal

Figure 2

From: Thiol-reactive reagents inhibits intracellular trafficking of human papillomavirus type 16 pseudovirions by binding to cysteine residues of major capsid protein L1

Figure 2

Infectivity of the 16PVs that have bound to BPEOIA, NEM, DTNB, MBTA, or MTSET. The 16PVs were incubated with the thiol-reactive reagent indicated at 37°C for 2 h. The samples were diluted by 1000-fold and added to HeLa cells. The cells were incubated for 2 days and harvested. The cells expressing EGFP were counted by a FACS. BPEOIA: biotin polyethyleneoxide iodoacetamid, NEM: N-ethylmaleimide, DTNB: 5,5'-dithiobis(2-nitrobenzoic acid), MBTA: 4-(N-maleimido)benzyl-trimethylammonium iodide, MTSET: [2-(trimethylammonium)ethyl] methanethiosulfonate bromide.

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Virology Journal

ISSN: 1743-422X