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Figure 4 | Virology Journal

Figure 4

From: Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein

Figure 4

Mapping of regions on NP involved in binding to VP35. (A) Schematic presentation of the constructed mutants of NP with C-terminal truncations. (B) The constructed plasmids were in vitro translated, metabolically labeled using [35S]ProMix, separated by SDS-PAGE and analyzed using a BioImager. (C) In vitro translated and metabolically labeled mutants of NP were incubated with bacterially expressed GST-VP35 or GST (negative control). Complexes were pulled down with glutathion-sepharose, separated on SDS PAGE and analyzed using a BioImager. Binding of NP to GST-VP35 was set to 100%. (D) Quantification of 3 separate experiments as shown under (C).

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Virology Journal

ISSN: 1743-422X