Skip to main content
Figure 5 | Virology Journal

Figure 5

From: Genetic incorporation of the protein transduction domain of Tat into Ad5 fiber enhances gene transfer efficacy

Figure 5

Competitive inhibition assay showing the enhanced gene transfer efficacy of Ad5.PTD tat was mediated by the PTD tat motif. In this assay, recombinant knob protein (50 μg/ml) was used to block CAR-mediated viral infection, and heparin (100 μg/ml) was used to block PTDtat mediated infection. Infections were performed at an MOI of 100 VPs/cell. (A) In low-CAR expressing U118MG cells that were resistant to unmodified Ad5 vector, Ad5.PTDtat mediated efficient gene delivery and the efficacy was completely inhibited by heparin, while recombinant knob had little effect, suggesting the enhanced infectivity of Ad5.PTDtat in low-CAR expressing cells resulted from the PTDtat motif. (B) In high-CAR expressing A549 cells, Ad5.PTDtat mediated gene delivery was partially inhibited with either knob or heparin, while being completely inhibited in the presence of both inhibitors, suggesting Ad5.PTDtat infected high-CAR expressing cells via both CAR and PTDtat pathways.

Back to article page