Figure 4

Ad5.PTD _tat exhibited enhanced gene transfer efficacy in a variety of tumor cells. Gene transfer efficacy was evaluated by use of a GFP reporter that was carried in the E1 region of each vector. In the assay, tumor cells expressing varying levels of CAR were infected with either Ad5 or Ad5.PTD_tat at an MOI of 100 or 500 VPs/cell, and GFP expression was examined by fluorescence microscopy and a fluorescence plate reader. (A) Representative fluorescence images of low-CAR containing cells (RD), medium-CAR containing cells (D65MG) and high-CAR expressing cells (Hela) that were infected with Ad5 or Ad5.PTD_tat at an MOI of 500 VPs/cell. (B) GFP expression in a variety of cells infected with either Ad5 or Ad5.PTD_tat was quantified using a fluorescence plate reader.