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Table 2 Plasimid Rescued influenza A & B Viruses In MDCK cellsa

From: Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells

Type Strain Virus titer (pfu/ml)
A   
   H1N1 ca A/New Caledonia/20/1999 7. 0 × 106
  ca A/Solomon Island/3/2006 5.4 × 107
   H2N2 MDV-A (ca A/Ann Arbor/6/1960) 1.5 × 108
   H3N2 ca A/Wisconsin/67/2005 8.4 × 106
  ca A/California/7/2004 2.6 × 106
  ca A/Panama/2007/1999 9. 0 × 106
   H5N1 ca A/Hong Kong/213/2003 1.3 × 107
  ca A/Hong Kong/1997(491 H5/486 N1) 1.7 × 107
  ca A/Vietnam/1203/2004 4.6 × 107
   H6N1 ca A/Teal/HK/W312/1997 6.0 × 106
   H7N3 ca A/CK/BC-CN/2004 3.9 × 106
   H9N2 ca A/chicken/HK/G9/1997 1.4 × 107
B   
  MDV-B (B/Ann Arbor/1/1966) 6.0 × 107
  MDV-B (B/Ann Arbor/1/1966)-Mutant 1.5 × 107
   Victoria ca B/Hong Kong/330/2001 1.7 × 107
  ca B/Malaysia/2506/2004 1.6 × 107
   Yamagata ca B/Jiangsu/10/2003 5.3 × 107
  ca B/Florida/07/2004 2.6 × 107
  1. a Supernatants of transfected MDCK cells were collected on days 5, 6, and 7 after transfection. Virus titer was determined by plaque assay for the supernatant collected on the day post-transfection when the MDCK cells exhibited 50 to 100% CPE.
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Virology Journal

ISSN: 1743-422X