|
Day post-transfection
|
Virus titer (pfu/ml)
|
|---|
| |
MDV-A
|
MDV-B
|
MDV-B (mutant)b
|
|---|
|
2
|
2.8 × 104
|
1.5 × 103
|
2.2 × 102
|
|
3
|
2.8 × 107
|
6.6 × 105
|
9.8 × 104
|
|
4
|
1.3 × 108
|
2.3 × 107
|
5. 2 × 106
|
|
5
|
3.8 × 107
|
1.9 × 107
|
1.8 × 107
|
|
6
|
1.2 × 107
|
3.6 × 106
|
3. 2 × 106
|
|
7
|
1.2 × 107
|
2.6 × 106
|
3. 0 × 106
|
- a Eight plasmids (3ug each) encoding the eight segments of MDV-A or MDV-B were combined with MDCK cells and subjected to electroporation. Virus titer (pfu/ml) of the supernatant was determined at the indicated days after electroporation by plaque assay on MDCK cells.
- b The MDV-B mutant was generated by substitution of MDV-B NS and PB1 plasmids containing silent mutations in their coding regions (NS 461A/G, PB1 561T/C, 924A/G) for their MDV-B counterparts in the eight plasmid MDV-B mix.
