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Table 1 Kinetics of MDVA and MDVB generation after transfectiona

From: Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells

Day post-transfection

Virus titer (pfu/ml)

 

MDV-A

MDV-B

MDV-B (mutant)b

2

2.8 × 104

1.5 × 103

2.2 × 102

3

2.8 × 107

6.6 × 105

9.8 × 104

4

1.3 × 108

2.3 × 107

5. 2 × 106

5

3.8 × 107

1.9 × 107

1.8 × 107

6

1.2 × 107

3.6 × 106

3. 2 × 106

7

1.2 × 107

2.6 × 106

3. 0 × 106

  1. a Eight plasmids (3ug each) encoding the eight segments of MDV-A or MDV-B were combined with MDCK cells and subjected to electroporation. Virus titer (pfu/ml) of the supernatant was determined at the indicated days after electroporation by plaque assay on MDCK cells.
  2. b The MDV-B mutant was generated by substitution of MDV-B NS and PB1 plasmids containing silent mutations in their coding regions (NS 461A/G, PB1 561T/C, 924A/G) for their MDV-B counterparts in the eight plasmid MDV-B mix.