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Table 1 Kinetics of MDVA and MDVB generation after transfectiona

From: Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells

Day post-transfection Virus titer (pfu/ml)
  MDV-A MDV-B MDV-B (mutant)b
2 2.8 × 104 1.5 × 103 2.2 × 102
3 2.8 × 107 6.6 × 105 9.8 × 104
4 1.3 × 108 2.3 × 107 5. 2 × 106
5 3.8 × 107 1.9 × 107 1.8 × 107
6 1.2 × 107 3.6 × 106 3. 2 × 106
7 1.2 × 107 2.6 × 106 3. 0 × 106
  1. a Eight plasmids (3ug each) encoding the eight segments of MDV-A or MDV-B were combined with MDCK cells and subjected to electroporation. Virus titer (pfu/ml) of the supernatant was determined at the indicated days after electroporation by plaque assay on MDCK cells.
  2. b The MDV-B mutant was generated by substitution of MDV-B NS and PB1 plasmids containing silent mutations in their coding regions (NS 461A/G, PB1 561T/C, 924A/G) for their MDV-B counterparts in the eight plasmid MDV-B mix.