Day post-transfection | Virus titer (pfu/ml) |
---|
 | MDV-A | MDV-B | MDV-B (mutant)b |
---|
2 | 2.8 × 104 | 1.5 × 103 | 2.2 × 102 |
3 | 2.8 × 107 | 6.6 × 105 | 9.8 × 104 |
4 | 1.3 × 108 | 2.3 × 107 | 5. 2 × 106 |
5 | 3.8 × 107 | 1.9 × 107 | 1.8 × 107 |
6 | 1.2 × 107 | 3.6 × 106 | 3. 2 × 106 |
7 | 1.2 × 107 | 2.6 × 106 | 3. 0 × 106 |
- a Eight plasmids (3ug each) encoding the eight segments of MDV-A or MDV-B were combined with MDCK cells and subjected to electroporation. Virus titer (pfu/ml) of the supernatant was determined at the indicated days after electroporation by plaque assay on MDCK cells.
- b The MDV-B mutant was generated by substitution of MDV-B NS and PB1 plasmids containing silent mutations in their coding regions (NS 461A/G, PB1 561T/C, 924A/G) for their MDV-B counterparts in the eight plasmid MDV-B mix.