Figure 3
From: Purification of infectious human herpesvirus 6A virions and association of host cell proteins
Metabolic labelling of proteins in HHV-6A. HHV-6A- or mock-infected cells were metabolically labelled with [35S]methionine between 24.5 and 28.5 hpi or 72.5 and 76.5 hpi and virions were collected without further labelling from 28.5 to 32.5 hpi or 76.5 to 80.5 hpi. HHV-6A virions and corresponding mock sample were purified, equalized based on the number of living cells in the two cultures at the end of collection period and analyzed by 6–15% SDS-PAGE. The 1 and 3 dpi media represented 2% of the total sample volume and fractions 11–15 corresponded to 98%. Estimated molecular weights in kD of the detected proteins are indicated. H, M and Mw abbreviations as in Fig. 3. Asterisks indicate cellular proteins 88 kD and 44 kD.