Infections of JJHAN cells with HHV-6A (U1102). A. Quantifications of cellular (diamonds) and extracellular (squares) HHV-6A DNA. The log10 of the viral DNA copy number per ml medium or per 106cells as normalized to actin, in inoculum, after 3 hpi and 1, 3, 5 and 7 dpi are shown. Results are based on three experiments and presented as mean ± standard deviation (error bars). B and C. Light microscopic analyses of HHV-6A- and mock-infected JJHAN cells at 3 dpi. Note the CPE, i.e. enlargement of cells, in HHV-6A infected cells. D and E. Detection of HHV-6 antigen gp60/110 in HHV-6A infected cells. HHV-6A-and mock-infected cells were fixed and stained for gp60/110 (red) and counterstained with DAPI (blue) to reveal cell nuclei. Light microscopy pictures are magnified 20× and fluorescent pictures 40×.