Figure 1

Transformed N. tabacum plants contain the transgene and are resistant to ToLCNDV. (Figure1 A) Southern hybridisation of EcoRI restricted DNA extracted from six primary transformed plants probed for AV2 sequences. Either 1 or 2 hybridising bands were detected in 5 of the 6 samples. (Figure1 B) Control N. tabacum plants inoculated with cloned ToLCNDV developed symptoms typical of ToLCNDV (left panel). Plants expressing the antisense-AV2 construct remained symptomless (right panel). Photographs were taken 3 weeks post-inoculation. (Figure1 C) Southern hybridisation of DNA extracted from control (lanes 1–6) and antisense-AV2 transformed (lanes 7–16) N. tabacum plants inoculated with cloned ToLCNDV. The blot was probed for the presence of the DNA A component of the virus. (Figure1 D) Serial dilutions of DNA extracted from three antisense-AV2 transformed plants (Lanes 1–12) and controls (Lanes 15–18) were tested for the presence of ToLCNDV by PCR. Dilution is made ten folds. In two transgenic lines the presence of ToLCNDV was detected only at higher template DNA concentrations.